Fig. 6.

In vivo ferroptosis-inducing study. A) Heatmap (A: saline group: B: t-ML@DGLA/siDECR1 ​+ ​M group) and B) volcano plots (red: PUFAs with a significant change, green: MUFAs with a significant change, gray: no significant change or saturated lipids) illustrating the levels of significantly regulated lipids in tumor tissues of t-ML@DGLA/siDECR1 ​+ ​M group when compared to normal saline group (n ​= ​6, |FC| ​> ​1.5, p ​< ​0.05, one-way ANOVA, values were expressed as log (FC)); C,D) Relative lipid amount change of PUFAs and MUFAs in saline (A) and t-ML@DGLA/siDECR1 ​+ ​M (B) groups (n ​= ​6, multiple t-test); E) QF-PCR analysis of DECR1 and GPX4 mRNA levels in tumors (n ​= ​3, mean ​± ​SD, multiple t-test, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001); F) Western blot and G) immunohistochemistry (IHC) analysis of DECR1 and GPX4 protein levels in tumors (scale bars ​= ​50 ​μm). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)