FIG. 6.

Increase of p50 protein in nuclei of tolerant Mono Mac 6 cells. Nuclei were generated by Dounce homogenization in the presence of a protease inhibitor cocktail followed by purification over a sucrose gradient. Extracts were separated by gel electrophoresis and blotted on nitrocellulose. Blots were stained with an anti-p65 or anti-p50 antibody and developed by using ECL. In the upper panel densitometry for p65 gave values of 3.0, 41.8, 6.2, and 44.9 for lanes 1 to 4, respectively (one of three experiments). The difference between lanes 3 and 4 was significant (P < 0.05). In the lower panel, densitometry for p50 gave values of 2.6, 6.9, 10.6, and 24.3 arbitrary units for lanes 1 to 4, respectively (one of three experiments). The difference between lanes 2 and 4 was significant (P < 0.05).