FIG. 7.

Effect of p50 overexpression on transactivation of the human TNF promoter. HEK 293 cells were calcium phosphate transfected with 0.5 μg of pTNF-1064 luci-β reporter plasmid and, as indicated with 0.25 μg of RcCMVp65 without or together with 2.5 μg of RcCMVp50 or RcCMV empty vector. Luciferase mRNA and enzyme activity were measured after 24 h. Plasmid-corrected mRNA values (A) were 3.0, 46.2, 21.9, and 103.3% for lanes 1 to 4; luciferase enzyme activities (B) were 66,193, 2,708,986, 538,750, and 6,960,601 relative light units for lanes 1 to 4. Data are representative of three and four experiments for mRNA and protein, respectively. The average fold reduction by p50 compared to empty plasmid was 6.1-fold for mRNA and 10.9-fold for enzyme activity (P < 0.01).