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. 2022 Oct 28;13:1000444. doi: 10.3389/fphar.2022.1000444

TABLE 1.

Primers used in qPCR.

Gene name Forward primer Reverse primer
FXR GCT​TGA​TGT​GCT​ACA​AAA​GCT​G CGT​GGT​GAT​GGT​TGA​ATG​TCC
BSEP TCT​GAC​TCA​GTG​ATT​CTT​CGC​A CCC​ATA​AAC​ATC​AGC​CAG​TTG​T
SHP TGG​GTC​CCA​AGG​AGT​ATG​C GCT​CCA​AGA​CTT​CAC​ACA​GTG
IL-6 TAG​TCC​TTC​CTA​CCC​CAA​TTT​CC TTG​GTC​CTT​AGC​CAC​TCC​TTC
ICAM1 GTG​ATG​CTC​AGG​TAT​CCA​TCC​A CAC​AGT​TCT​CAA​AGC​ACA​GCG
COX-2 TTC​AAC​ACA​CTC​TAT​CAC​TGG​C AGA​AGC​GTT​TGC​GGT​ACT​CAT
ZO-1 GCC​GCT​AAG​AGC​ACA​GCA​A TCC​CCA​CTC​TGA​AAA​TGA​GGA
occludin TTG​AAA​GTC​CAC​CTC​CTT​ACA​GA CCG​GAT​AAA​AAG​AGT​ACG​CTG​G
acludin-1 GGG​GAC​AAC​ATC​GTG​ACC​G AGG​AGT​CGA​AGA​CTT​TGC​ACT
h FXR AAC​CAT​ACT​CGC​AAT​ACA​GCA​A ACA​GCT​CAT​CCC​CTT​TGA​TCC
h SHP GTG​CCC​AGC​ATA​CTC​AAG​AAG TGG​GGT​CTG​TCT​GGC​AGT​T
h BSEP TTG​GCT​GAT​GTT​TGT​GGG​AAG CCA​AAA​ATG​AGT​AGC​ACG​CCT
β-actin ATG​ACC​CAA​GCC​GAG​AAG​G CGG​CCA​AGT​CTT​AGA​GTT​GTT​G
h β-actin GAA​TCA​ATG​CAA​GTT​CGG​TTC​C TCA​TCT​CCG​CTA​TTA​GCT​CCG