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. 2022 Nov 10;12:19239. doi: 10.1038/s41598-022-23831-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs of semithin sections of the earthworms (Lumbricus castaneus) (a) normal earthworm; (b) worms received Vaseline, showing fissure(raw); (c) worm on the first day of injured showing the coelomic fluid emerged as well as the blood surrounds the wound appearance (circle), and (d) worms received 5 mg, (e) worms received 10 mg showing fissure(raw), (f) worms received 15 mg. Cm Circular muscle, Ep Epidermis, Lm Longitudinal muscle, Wo Wound.