KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-mouse CD45.2 Clone 104 (Pac Blue); 1:10 dilution IN | Biolegend | Cat 109820 |
| α-mouse CD45 Clone 30-F11 (BUV395); 1:500 dilution | BD | Cat 564279 |
| α-mouse B220 Clone RA3-6B2 (BV-711); 1:500 dilution | Biolegend | Cat 103255 |
| α-mouse IgG Poly4053 (APC); 1:250 dilution | Biolegend | Cat 405308 |
| α-mouse IgA Clone C10-3 (FITC); 1:250 dilution | BD | Cat 559354 |
| α-mouse IgM Clone RMM-1 (PE-Cy7); 1:250 dilution | Biolegend | Cat 406513 |
| Zombie Viability UV Dye; 1:1000 | Biolegend | Cat 423107 |
| α-mouse EpCAM Clone G8.8 | BioXCell | Cat BE0346 |
| α-rat-AlexaFluor647 | Jackson Immunoresearch | Code 712-605-153 |
| α-mouse CXCR3 Clone CXCR3-173 | BioXCell | Cat BE0249 |
| α-mouse CD4 Clone GK1.5 | BioXCell | Cat BE0003-1 |
| α-mouse CD40L Clone Mr-1 | BioXCell | Cat BE0017-1 |
| α-mouse CD8 Clone YTS 169.4 | BioXCell | Cat BE0117 |
| α-mouse NK1.1 Clone PK136 | BioXCell | Cat BE0036 |
| Bacterial and virus strains | ||
| VSV ovalbumin, serotype Indiana | (Kim et al., 1998) | N/A |
| VSV ovalbumin, serotype New Jersey | (Kim et al., 1998) | N/A |
| VSV-Sars-S-GFP | (Case et al., 2020) | SRA: SRR11878607 |
| Influenza B/Malaysia/2506/2004 (Mal/04) | N/A | NCBI:txid464417 |
| Mal/04-Cre | (Dumm et al., 2019) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| VI10 anti-VSV mAb | (Kalinke et. al., 1996) | N/A |
| Bortezomib | Millipore | CAS 179324-69-7 |
| Marizomib | Sigma | SKU SML1916 |
| Imject Alum | ThermoFisher | Cat: 77161 |
| CpG ODN 2395 | Invivogen | Cat: tlrl-2395 |
| dmLT (Enterotoxigenic E. coli Double Mutant Heat-Labile Toxoid) | BEI Resources | NR-51683 |
| SARS-CoV-2 mRNA vaccine | Pfizer-BioNTech | N/A |
| Critical commercial assays | ||
| QIAamp Viral RNA Isolation Kit | Qiagen | #52904 |
| Express One-Step Superscript qRT-PCR Kit | ThermoFisher | #1178101 |
| Deposited Data | ||
| Previously published single-cell RNA sequencing data | (Durante et. al., 2020) | GEO accession code: GSE139522 |
| Experimental models: Cell lines | ||
| Monkey: Vero cells | ATCC | CCL-81 |
| Monkey: Vero E6 cells | ATCC | CRL-1586 |
| Canine: MDCK cells | ATCC | CCL-34 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J (B6) | Jackson Laboratories | RRID:IMSR_JAX:000664 |
| Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (mT/mG) | Jackson Laboratories | RRID:IMSR_JAX:007676 |
| Mouse: B6.129P2-Cxcr3tm1Dgen/J (Cxcr3−/−) | Jackson Laboratories | RRID:IMSR_JAX:005796 |
| Mouse: B6.129S2-Ighmtm1Cgn/J (μMT) | Jackson Laboratories | RRID:IMSR_JAX:002288 |
| Mouse: B6;129S2-Tcratm1Mom/J (Tcra−/−) | Jackson Laboratories | RRID:IMSR_JAX:002116 |
| Mouse: B6.129P2-Aicdatm1(cre)Mnz/J (AIDCre/+, AID−/−) | Jackson Laboratories | RRID:IMSR_JAX:007770 |
| Mouse: C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J (iDTR) | Jackson Laboratories | RRID:IMSR_JAX:007900 |
| Mouse: B6.Cg-Tg(K18-ACE2)2Prlmn/J (K18) | Jackson Laboratories | RRID:IMSR_JAX:034860 |
| Mouse: B6;129P2-Omptm3Mom/MomJ (OMP-GFP) | Jackson Laboratories | RRID:IMSR_JAX:00666 |
| Oligonucleotides | ||
| Cxcl9 FWD: AGGCACGATCCACTACAAATC (500 nM) | This paper | N/A |
| Cxcl9 REV: GCAGGTTTGATCTCCGTTCT (500 nM) | This paper | N/A |
| Cxcl9 Probe: /56-FAM/CCTCAAAGA/ZEN/CCTCAAACAGTTTGCCC/3IABkFQ/ (200 nM) | This paper | N/A |
| Cxcl10 FWD: GGCCATAGGGAAGCTTGAAA (500 nM) | This paper | N/A |
| Cxcl10 REV: CAGACATCTCTGCTCATCATTCT (500 nM) | This paper | N/A |
| Cxcl10 Probe: /5SUN/TCCTGCCCA/ZEN/CGTGTTGAGATCATT/3IABkFQ/ (200 nM) | This paper | N/A |
| Eukaryotic 18s rRNA Endogenous Control | ThermoFisher | # CCU002NR |
| SARS CoV-2 S FWD: ACAGGCACAGGTGTTCTTAC (500 nM) | This paper | N/A |
| SARS CoV-2 S REV: GATCACGGACAGCATCAGTAG (500 nM) | This paper | N/A |
| SARS CoV-2 S Probe: /56-FAM/CTGCCTTTC/ZEN/CAACAATTTGGCAGAGAC/3IABkFQ/ | This paper | N/A |
| Software and algorithms | ||
| Imaris v7.6.1 | Bitplane | N/A |
| Flowjo v10.8.1 | BD | N/A |
| R v4.0.3 | R Project | N/A |
| Seurat v4.1.0 | Satija Lab | N/A |
| Prism v9.1.2 | GraphPad | N/A |
| Complete code for analysis of single-cell RNA sequencing data | This paper | DOI: 10.5281/zenodo.7015296 https://zenodo.org/badge/latestdoi/474683647 |