Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD103 | BD Biosciences | M290 |
| Anti-mouse CD11b | BD Biosciences | M1/70 |
| Anti-mouse CD11c | BD Biosciences | N418 |
| Anti-mouse CD4 | BD Biosciences | RM4-5 |
| Anti-mouse CD45 | BD Biosciences | 30-F11 |
| Anti-mouse Ki67 | BD Biosciences | B56 |
| Anti-mouse Ly6C | BD Biosciences | AL-21 |
| Anti-mouse MHC-II I-A/I-E | BD Biosciences | M5/114.15.2 |
| Anti-mouse SiglecF | BD Biosciences | E50-2440 |
| Anti-human CD141 | BD Biosciences | 1A4 |
| Anti-human CD1c | BD Biosciences | F10/21A3 |
| Anti-mouse CD206 | BioLegend | C068C2 |
| Anti-mouse H-2Kb bound to SIINFEKL | BioLegend | 25-D1.16 |
| Anti-mouse XCR1 | BioLegend | ZET |
| Anti-human CD15 | BioLegend | W6D3 |
| Anti-human CD16 | BioLegend | 3G8 |
| Anti-human CD19 | BioLegend | HIB19 |
| Anti-mouse CD29 | BioLegend | HmB1-1 |
| Anti-mouse CD326 (EpCAM) | BioLegend | G8.8 |
| Anti-human CD56 | BioLegend | HCD56 |
| Anti-human HLA-DR | BioLegend | L243 |
| Anti-mouse CD24 | eBioscience/Thermo | M1/69 |
| Anti-mouse CD80 | eBioscience/Thermo | 16-10A1 |
| Anti-mouse CD86 | eBioscience/Thermo | GL1 |
| Anti-mouse FoxP3 | eBioscience/Thermo | FJK-16s |
| Anti-mouse NK1.1 | eBioscience/Thermo | PK136 |
| Anti-mouse PD-L1 | eBioscience/Thermo | MIH5 |
| Anti-mouse PD-1 | eBioscience/Thermo | RMP1-30 |
| Anti-mouse TIM-3 | eBioscience/Thermo | 8B.2C12 |
| Anti-mouse Vcam1 | eBioscience/Thermo | 429 |
| Anti-mouse/human IRF8 | eBioscience/Thermo | V3GYWCH |
| Anti-human CD14 | eBioscience/Thermo | 61D3 |
| Anti-human/mouse granzyme B | Invitrogen | GB11 |
| Anti-human CD3d | Invitrogen | 7D6 |
| CellTrace Violet | Invitrogen | |
| Anti-mouse B220 | Tonbo Biosciences | RA3-6B2 |
| Anti-mouse CD8a | Tonbo Biosciences | 53-6.7 |
| Anti-mouse Ly6G | Tonbo Biosciences | 1A8 |
| Anti-mouse TCRB | Tonbo Biosciences | H57-597 |
| Anti-human CD45 | Tonbo Biosciences | 2D1 |
| Ghost Dye | Tonbo Biosciences | 13-0865-T500 13-0863-T500 13-0870-T500 |
| CD8 depleting antibody | Bio × Cell | Clone 2.43 |
| FcR Block (2.4G2 mAB) | Bio × Cell | 25 mg (custom) |
| Human TruStain FcX | BioLegend | 422302 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Collagenase Type 3 | Worthington Biochemical | LS004183 |
| Dnase I | Sigma-Aldrich | |
| Percoll | Sigma-Aldrich | P1644-1L |
| Trizol LS | Invitrogen | |
| OVA(257–264) | Invivogen | |
| Critical Commercial Assays | ||
| Transcription factor Fix/Perm Kit | Tonbo Biosciences | TNB-0607-KIT |
| Naïve CD8 T cell Negative Selection Kit | EasySep, Stem Cell Technologies | 19858 |
| Deposited Data | ||
| Murine bulk RNAseq/scRNAseq sequencing files | GEO | GSE212643 |
| Human TAM monocyte bulk RNAseq sequencing files | EGA | Dataset ID: EGAD00001009393 Study ID: EGAS00001006593 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.Cg-Tg(Itgax-cre)1-1Reiz/J (CD11cCre) (Received from Dr. Boris Reizis Lab) | Jackson Laboratories | Stock: 008068 |
| Mouse: B6.SJL-PtprcaPepcb/BoyJ (CD45.1) | Jackson Laboratories | Stock: 002014 |
| Mouse: B6(Cg)-Irf8tm1.1Hm/J (Irf8flox) | Jackson Laboratories | Stock: 014175 |
| Mouse: B6(Cg)-Irf8tm2.1Hm/J(Irf8EGFP) (Received from Dr. Herbert C. Morse III Lab) | Jackson Laboratories | Stock: 027084 |
| Mouse: B6N(129S4)-Mafbtm1.1(cre)Kmm/J (MafBiCre) (Received from Dr. Kenneth Murphy Lab) | Jackson Laboratories | Stock: 029664 |
| Mouse: C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I) | Jackson Laboratories | Stock: 003831 |
| Mouse: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ (PyMT) | Jackson Laboratories | Stock: 022974 |
| Mouse: B6.Cg-Tg(S100A8-Cre,-EGFP) 1Ilw/J (S100a8Cre) | Jackson Laboratories | Stock: 021614 |
| USA-LSL (“Universal Self Antigen”, Received from Dr. James J. Moon Lab) | Zhang Z. et al, 2019 Eur J Immunol. | N/A |
| Mouse: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J (Rosa26LSL-YFP) | Jackson Laboratories | Stock: 006148 |
| Software and Algorithms | ||
| GraphPad Prism V.7-9 | GraphPad | |
| FlowJo V.9, V.10 | TreeStar | |
| FacsDIVA | BD Biosciences | |
| R software | ||
| Other | ||