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. 2022 Aug 30;28:100400. doi: 10.1016/j.pacs.2022.100400

Fig. 6.

Fig. 6

Early diagnosis of bladder cancer. A) Velocity of urine in unshake and shake condition (one representative experiment of four). B) Velocity of GNRs@Chit-Iso4 (3 nmol Au) in unshake and shake condition (one representative experiment of four). C) 3D distribution of the GNRs@Chit-Iso4 (3 nmol Au) PA signal in murine bladder in the unshake and shake condition (one representative experiment of four); the green signal corresponds to the PA signal of GNRs after unmixing the PA signal of melanin, deoxy- and oxy-genated blood and GNRs. D) Axial frame of a murine bladder with an US recognizable tumor nine days after intravesical instillation of MB49-Luc cells (one representative experiment of six); axial diameter of the bladder lumen = 4.8 mm. E) Axial frame of PAI of the same animal shown in panel D after the intravesical instillation of GNRs@Chit-Iso4 (3 nmol Au), followed by 3 cycles of shaking, removal of the GNRs and 2 washes with saline solution (one representative experiment of six); axial diameter of the bladder lumen = 3.7 mm. F) PAI of the same animal used in panel D-E 5 days after the instillation of the GNRs@Chit-Iso4 (one representative experiment of six); axial diameter of the bladder lumen = 4.2 mm. G) Time course bioluminescence of 12 animals without or with orthotopic bladder tumors derived from intravesical instillation of MB49-Luc cells (mean±SEM); the arrow indicates the day when the PAI was acquired to diagnosis the orthotopic bladder tumor in the experiment reported in panel H-M. Axial (H; diameter of bladder lumen= 5.5 mm) and longitudinal (I; diameter of bladder lumen = 11 mm) frames and 3D reconstruction (J) of US imaging of the murine bladder 4 days after the intravesical instillation of MB49-Luc cells (one representative experiment of four).

Axial (K; diameter of bladder lumen = 6.5 mm), longitudinal (L; diameter of bladder lumen = 11 mm) and 3D distribution (M) of the GNRs@Chit-Iso4 (3 nmol Au) PA signal in the murine bladder with tumor not detectable by US imaging and bioluminescence (day 4 after intravesical instillation of MB49-Luc cells) (one representative experiment of four). Axial (N; diameter of bladder lumen = 6.2 mm) and longitudinal (O; diameter of bladder lumen = 12 mm) frames, and 3D reconstruction (P) of US imaging of bladder and orthotopic tumor three days after tumor recognition by GNRs@Chit-Iso4 (one representative experiment of four). Vol: Volume.