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Cytocompatibility of DCS-RuB2A2. The CCK-8 method detected the cytotoxicity of CS and DCS with differing degrees of substitution (A, B) and RuB2A2 at different concentrations (C, D). (E, F) CCK-8 assays for the biocompatibility of DCS-RuB2A2 under light and dark conditions. (G) (PI/Hoechst) live and dead cell staining images and statistical analysis of HEK 293T cells cultured for 24 h, scale bars 50 μm (n ≥ 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
