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. 2022 Oct 20;25(11):105425. doi: 10.1016/j.isci.2022.105425

Figure 7.

Figure 7

RtcB-mediated repair targets the tmRNA ssrA under oxidative stress

Targets of the RtcB ligase, including the tmRNA ssrA, were identified by RtcB mediated ligation using an adapter and followed by NGS.

(A) Number of genes whose RNA is an RtcB ligase target (left) and events of RtcB-mediated ligations (right).

(B) Ligation events for ssrA in wild-type (green arrows) and Hpx- cells (red arrows). Different colours indicate distinct ssrA structural domains, including the tRNA-like domain (TLD), the mRNA-like domain (MLD), helices 2 (H2) and 5 (H5), and pseudoknots 1 (PK1), 2 (PK2), 3 (PK3) and 4 (PK4). The nucleotides of the open reading frame (ORF) are shown in a larger font, whereas the resume and stop codons are highlighted. Ligation events were significantly more abundant in PK3 (p-value < 0.01) for wild-type cells, and in both PK3 (p-value < 0.01) and PK4 (p-value < 0.0001) for Hpx- cells.