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. 2022 Oct 28;13:932761. doi: 10.3389/fendo.2022.932761

Figure 5.

Figure 5

LEAP2 overexpression in the ARC increases the hypothalamic POMC expression but no change of AGRP, NPY, and GHSR in mice fed chow or a high-fat diet. (A) Relative mRNA levels of Agrp, Npy, Pomc, and Ghsr in the hypothalamus of mice fed a chow diet and injected with AAV-GFP or AAV-LEAP2 for 12 weeks. n = 6/group, two-tailed Student’s t-test, t = 4.481, ** p = 0.0037< 0.01 for Pomc. (B, C) Representative immunoblot images of proteins (B) and densitometric quantification (C) for AGRP, POMC, and GHSR in the hypothalamus of mice fed a chow diet after virus injection for 12 weeks. α-Tubulin was used as the loading control. n = 4–6/group, two-tailed Student’s t-test, t = 5.490, ** p = 0.0014< 0.01 for POMC (C). (D) Relative mRNA levels of Agrp, Npy, Pomc, and Ghsr in the hypothalamus of mice fed a HFD and injected with AAV-GFP or AAV-LEAP2 for 12 weeks. n = 6/group, two-tailed Student’s t-test, t = 3.513, ** p = 0.0056< 0.01 for Pomc. (E, F) Representative immunoblot images of proteins (E) and densitometric quantification (F) for AGRP, POMC, and GHSR in the hypothalamus of mice fed a HFD after virus injection for 12 weeks. α-Tubulin was used as the loading control. n = 4–6/group, two-tailed Student’s t-test, t = 6.281, *** p = 0.0002< 0.001 for POMC (F). Data are presented as mean ± SEM. ** p< 0.01; *** p< 0.001.