TABLE 1.
Primary antibody | Secondary antibody | |
pDRP1 Ser616 (78–82 kDa) | 1:1000 BSA 3% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 BSA3%, 1h RT |
DRP1 (78–82 kDa) | 1:1000 BSA 3% (Cell signaling), 4° O/N | Anti-mouse 1:1000 BSA3%, 1h RT |
OPA1 (80 kDa) | 1:1000 BSA 3% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 BSA3%, 1h RT |
MFN2 (80 kDa) | 1:1000 BSA 3% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 BSA3%, 1h RT |
OXPHOS (55–40–20 KDa) | 1:2000 BSA 3% (Abcam), 4° O/N | Anti-mouse 1:2000 BSA3%, 1h RT |
SIRT1 (120 Kda) | 1:1000 BSA 5% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 BSA5%, 1h RT |
PGC1a (91 Kda) | 1:1000 BSA 5% (Novus), 4° O/N | Anti-rabbit 1:1000 BSA5%, 1h RT |
CAT (60 KDa) | 1:1000 M3% (Cell signaling), 4° O/N | Anti-rabbit 1:1000 M3%, 1h RT |
β-ACTIN (43 kDa) | 1:10000 M3% (Sigma-Aldrich), 4° O/N | Anti-mouse 1:10000 M3%, 1h RT |
BSA, Bovin serum albumin; M, Milk; O/N, overnight; RT, room temperature.