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. 2022 Oct 28;16:957702. doi: 10.3389/fnbeh.2022.957702

TABLE 1.

Antibodies conditions used in the western blot.

Primary antibody Secondary antibody
pDRP1 Ser616 (78–82 kDa) 1:1000 BSA 3% (Cell signaling), 4° O/N Anti-rabbit 1:1000 BSA3%, 1h RT
DRP1 (78–82 kDa) 1:1000 BSA 3% (Cell signaling), 4° O/N Anti-mouse 1:1000 BSA3%, 1h RT
OPA1 (80 kDa) 1:1000 BSA 3% (Cell signaling), 4° O/N Anti-rabbit 1:1000 BSA3%, 1h RT
MFN2 (80 kDa) 1:1000 BSA 3% (Cell signaling), 4° O/N Anti-rabbit 1:1000 BSA3%, 1h RT
OXPHOS (55–40–20 KDa) 1:2000 BSA 3% (Abcam), 4° O/N Anti-mouse 1:2000 BSA3%, 1h RT
SIRT1 (120 Kda) 1:1000 BSA 5% (Cell signaling), 4° O/N Anti-rabbit 1:1000 BSA5%, 1h RT
PGC1a (91 Kda) 1:1000 BSA 5% (Novus), 4° O/N Anti-rabbit 1:1000 BSA5%, 1h RT
CAT (60 KDa) 1:1000 M3% (Cell signaling), 4° O/N Anti-rabbit 1:1000 M3%, 1h RT
β-ACTIN (43 kDa) 1:10000 M3% (Sigma-Aldrich), 4° O/N Anti-mouse 1:10000 M3%, 1h RT

BSA, Bovin serum albumin; M, Milk; O/N, overnight; RT, room temperature.