| Time (min) | Flow rate (ml/min) | %B |
| Initial | 1.0 | 15 |
| 27.0 | 1.0 | 45 |
| 37.5 | 1.0 | 80 |
| 50.0 | 1.0 | 80 |
| 51 | 1.0 | 15 |
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