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. 2022 Oct 28;13:1017385. doi: 10.3389/fimmu.2022.1017385

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Copyright © 2022 Galloway, Nguyen, Li, Houston, Gregersen, Williamson, Falkenberg, Herron and Hale

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Germinal center B cell responses 7 days post-immunization. Analysis of lymphocyte responses in the draining lymph nodes (dLNs) 7 days post-immunization. (A) Total number of CD19+ cells in dLNs. (B) Flow cytometry analysis of CD19+ gated B cells with gate showing PNA+Fas+ GC B cells. (C) Frequency and (D) number of GC B cells. n=5 per group per experiment at each time point. Data shown are from one experiment and are representative of two independent experiments. Statistically significant p values were determined using a two-tailed unpaired Student t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001..