Fig. 1. Overview of methodological approaches to isolate EV for proteomics. Density gradient ultracentrifuge is considered the gold-standard method in EV proteomics. The purity of EVs could be estimated by particle number per protein amount as suggested by Webber and Clayton [37]. EV, extracellular vesicle; CSF, cerebrospinal fluid; NTA, nanoparticle tracking analysis; TRPS, tunable resistive pulse sensing.
