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. 1999 Apr;67(4):1599–1605. doi: 10.1128/iai.67.4.1599-1605.1999

FIG. 1.

FIG. 1

Effect of neutralizing anti-IL-10 MAb on ConA-driven responses of spleen cells from mice infected with L3. Mice were injected s.c. with 50 L3 or HBSS, and spleens were removed at day 12 p.i. ConA-stimulated cultures were incubated alone (□) or with 10 μg of either an isotype-matched control (R59-40) (▨) or anti-IL-10 (JES5-2A5) ( Created by potrace 1.16, written by Peter Selinger 2001-2019 ) per ml. After 48 h of incubation, proliferation (A) and IL-2 (B) and IFN-γ (C) production were measured. (A) The means of triplicate wells are expressed as SIs (cpm with ConA/cpm with medium alone). The SIs represent the means ± standard deviations of five animals per group, ∗, significant difference (P < 0.05) between the values obtained with JES5-2A5 and R59-40. SIs were also significantly different (P < 0.05) between the cells of L3-infected mice treated with JES5-2A5 and uninfected mice with no treatment. (B) and (C) Cytokine assays were performed with spleen cells pooled from five animals in each group. The results presented were comparable in two additional experiments.