TABLE 1.
Group | Mean % positive ± SEMb
|
||
---|---|---|---|
CD11c+ class II− | CD11c+ class II+ | CD11c− class II+ | |
Day 0 | 0 | 4.1 ± 1.0 | 19.8 ± 3.6 |
Day 3 | |||
DEB | 0 | 0.3 ± 0.2 | 5.3 ± 1.6 |
LEB | 8.2 ± 1.5c | 6.9 ± 2.5c | 11.8 ± 2.9 |
Day 7 | |||
DEB | 0 | 3.2 ± 0.75 | 21.2 ± 8.6 |
LEB | 0 | 13.1 ± 0.45 | 20.3 ± 5.8 |
Day 21 | |||
DEB | 0 | 0.75 | 29.7 |
LEB | 0 | 0.4 | 43.0 |
Four mice for each group were studied on days 0, 3, and 7 postimmunization. One mouse from each group was studied at day 21. Mice were immunized intraperitoneally with dead (UV-killed) or live MoPn EB (DEB or LEB). The cells were double stained with PE-conjugated anti-CD11c and FITC-conjugated anti-MHC class II and analyzed by flow cytometry.
Percentage of surface marker-positive cells as a proportion of total viable peritoneal cell population. Propidium iodine staining and scatter gating were used to exclude dead cells.
P < 0.05 compared with the group of mice immunized with dead EB.