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. 1999 Apr;67(4):1606–1613. doi: 10.1128/iai.67.4.1606-1613.1999

TABLE 1.

Distribution of CD11c and MHC class II markers on peritoneal cells obtained from naive mice immunized with live and dead C. trachomatis MoPn EBsa

Group Mean % positive ± SEMb
CD11c+ class II CD11c+ class II+ CD11c class II+
Day 0 0 4.1 ± 1.0 19.8 ± 3.6
Day 3
 DEB 0 0.3 ± 0.2 5.3 ± 1.6
 LEB 8.2 ± 1.5c 6.9 ± 2.5c 11.8 ± 2.9
Day 7
 DEB 0 3.2 ± 0.75 21.2 ± 8.6
 LEB 0 13.1 ± 0.45 20.3 ± 5.8
Day 21
 DEB 0 0.75 29.7
 LEB 0 0.4  43.0
a

Four mice for each group were studied on days 0, 3, and 7 postimmunization. One mouse from each group was studied at day 21. Mice were immunized intraperitoneally with dead (UV-killed) or live MoPn EB (DEB or LEB). The cells were double stained with PE-conjugated anti-CD11c and FITC-conjugated anti-MHC class II and analyzed by flow cytometry. 

b

Percentage of surface marker-positive cells as a proportion of total viable peritoneal cell population. Propidium iodine staining and scatter gating were used to exclude dead cells. 

c

P < 0.05 compared with the group of mice immunized with dead EB.