Figure 2.

Development and morphology of OsDREB2B-OE rice. (A) Schematic representation of OsDREB2B plant expression vector. OsDREB2B was expressed under the control of maize ubiquitin (Ubi) promoter, and the hygromycin (hgy) gene was under the control of 35S promoter as a selection marker in rice. (B) PCR analyses for T₀ transgenic rice using construct-specific primers. M, marker; WT, Kitaake; 1~18, independent transformants of T₀ generation. (C) The expression of OsDREB2B were analyzed by qRT-PCR in WT and DE. The data was presented as the mean ± SE values (n = 3) from two independent experiments. (D, E) Whole-plant morphology of the OE lines and the WT at the seedling stage. Bar = 2 cm. (F, G) Whole plant morphology of OsDREB2B-OE plants and the WT at maturity. Bar = 10 cm. (H) Panicles and internodes of the main culms of OE-1 (right) and the WT (left). Bar = 5 cm. (I) Internode lengths of OE lines and the WT. (J) Seed morphology. Bar = 1 cm. (K) Seed size of the OE lines and the WT. Single asterisk indicates significant differences at P ≤ 0.05 compared with the WT and double asterisks indicate significant differences at P ≤ 0.01 compared with the WT in Student’s t-test.