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. 2022 Oct 28;13:1007811. doi: 10.3389/fpls.2022.1007811

Figure 8.

Figure 8

Direct regulation of OsAP2-39 by OsDREB2B. (A) Relative expression of the OsAP2-39 gene in the WT and OE lines. (B) Combination of OsDREB2B and OsAP2-39 in yeast. GAD-OsDREB2B activates the expression of LacZ reporter genes driven by OsAP2-39 promoters in yeast. GAD, GAL4 transcriptional activation domain. (C) Schematic diagram of the carrier in the dual luciferase reporter system. (D) In vivo dual luciferase reporter assay. Flag-OsDREB2B and OsAP2-39pro:FLUC, Flag, and OsAP2-39pro:FLUC were co-transformed into rice protoplasts and incubated at 28°C for 12–14 (h) FLuc and RLuc activities were measured, and Renilla luciferase (RLuc) was used as an internal control. Student’s t-tests were used to generate the P values. **Significant differences between the WT and OE plants at P = 0.01.