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. 2022 Oct 28;13:1001956. doi: 10.3389/fimmu.2022.1001956

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Copyright © 2022 Ogbechi, Huang, Clanchy, Pantazi, Topping, Darlington, Williams and Stone

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PQS inhibits IDO1 expression in human M1 macrophages. Monocyte-derived macrophages were polarised to an M1 phenotype overnight in the presence or absence of PQS (10µM) to measure gene expression, IDO1 protein expression and IDO1 activity. (A) Gene expression of IL1β, IL6, TNF, IDO1 and IDO2; n=3 donors, *p<0.05. (B) Western blots of IDO1 and β-actin for 3 independent donors A, B and C. (C) (left) -quantification of (B); n=3 donors, *p<0.05; (right) - measurement of kynurenine in the culture medium (ND, not detected); n=3 donors.