FIG. 5.

Examination by 2-D gel electrophoresis of S. typhimurium proteins affected by bile. (A) Wild-type cells grown in LB broth. (B) Cells grown in LB broth plus 3% bile. Protein species in circles denote those repressed by bile, and protein species in squares denote those activated by bile. Only those proteins most obviously affected by bile are noted. The arrowhead indicates the isoelectric focusing standard (molecular mass, 33 kDa; pI 5.2). Molecular mass standards (in kilodaltons) are noted to the left of each panel.