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BCAA metabolism contributes to OA-induced hepatic steatosis. (A–F) LO2 and HepG2 cells were incubated with 0.5 mm Oleic acid with or without 10 mm BCAAs for 48 h. Intracellular lipid droplets were detected by Oil red O staining. Scale bar = 400 μM (A,B). Intracellular TC and TG levels in LO2 (C,D) and HepG2 (E,F) cells were detected. Data are shown as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001.
