FIGURE 5.

BCATc Inhibitor 2 alleviates OA-induced cell apoptosis. (A,B) LO2 and HepG2 cells were incubated with 0.5 mm Oleic acid with or without 10 mm BCAAs for 48 h. Cell viability was detected by MTT assay. (C,D) LO2 and HepG2 cells were incubated with 0.5 mm Oleic acid in the presence 0, 10, 20 µM BCAT-IN-2 for 48 h. Cell viability was detected by MTT assay. (E) LO2 cells were incubated with 0.5 mm Oleic acid with or without 10 mm BCAAs for 48 h. Apoptotic cell rate was determined by Annexin V/PI staining and cytometry based on Q2+Q3. (F) LO2 cells were incubated with 0.5 mm Oleic acid and 0, 10, 20 µM BCATc Inhibitor 2 for 48 h. Apoptotic cell rate was determined by Annexin V/PI staining and cytometry based on Q2+Q3. Data are shown as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001. INc: BCATc Inhibitor 2.