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. 2022 Oct 28;13:1006413. doi: 10.3389/fpls.2022.1006413

Table 5.

Leaf mass per area (LMA) and pigments in leaves of fullmoon maple seedlings grown under different light conditions, sampled in the morning of Oct 19.

Oct 19 Open Shade
H0 L0 LH1 LH2 LH3 F-statistics
LMA (g m-2) 62.5 ± 3.6 a 35.4 ± 1.2 b 37.0 ± 1.8 b 34.4 ± 0.7 b 39.4 ± 3.0 b 23.4 *** (F4,17)
Chl a+b
(µmol g-1)
2.98 ± 0.39 b 8.55 ± 1.06 a 6.70 ± 0.32 ab 6.54 ± 0.67 ab 5.87 ± 1.17 ab 4.63 * (F4,17)
Chl a:b 2.55 ± 0.09 2.65 ± 0.14 2.21 ± 0.12 2.38 ± 0.12 2.65 ± 0.07 2.30 ns (F4,17)
(V+A+Z)/Chl
(mmol mol-1)
154.2 ± 16.9 a 57.7 ± 9.4 b 94.2 ± 5.1 b 88.5 ± 11.6 b 69.0 ± 11.9 b 9.96 *** (F4,17)
(Z+A)/(V+A+Z) 0.79 ± 0.05 a 0.57 ± 0.06 b 0.88 ± 0.02 a 0.81 ± 0.03 a 0.78 ± 0.02 a 6.76 ** (F4,17)
Anthocyanin/Chl
(mol mol-1)
2.32 ± 0.65 a 0.63 ± 0.34 b 1.87 ± 0.10 ab 0.91 ± 0.19 ab 0.58 ± 0.29 b 4.14 * (F4,17)

Pigments analyzed were dry-mass based leaf total chlorophyll content (Chl a+b), chlorophyll a to b ratio (Chl a:b), chlorophyll-based xanthophyll cycle pigments content ((V+A+Z)/Chl), including violaxanthin (V), antheraxanthin (A), and zeaxanthin (Z), conversion state of xanthophyll cycle ((Z+A)/(V+A+Z)), chlorophyll-based anthocyanins content (anthocyanin/Chl). H0: open condition, L0: shade condition (relative irradiance of ≈ 13% of open condition) throughout the experimental period, LH1: transfer from shade to open on Oct 5, LH2: transfer from shade to open on Oct 12, and LH3: transfer from shade to open on Oct 18. Values are means ± se; n= 3 to 6. *, **, and *** denote significant effect at P ≤ 0.05, ≤ 0.01, and ≤ 0.001, respectively. ns indicates no statistically significant effect. Different letters indicate significant differences among means of light treatments on the same date at P < 0.05.