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. 2022 Oct 28;13:1024639. doi: 10.3389/fmicb.2022.1024639

Figure 5.

Figure 5

Binding affinity analysis of IPTG with SotB/SotB variants. (A) Binding affinity of IPTG with SotB; (B) Binding affinity of IPTG with E29A; (C) Binding affinity of IPTG with H115A; (D) Binding affinity of IPTG with W119A; (E) Binding affinity of IPTG with S339A; (F) Binding affinity of IPTG with N343A. The colored lines represent the concentrations of IPTG used in SPR assays; (G) Coordination of IPTG by SotB. Hydrogen bonds are represented with yellow dashed lines. IPTG and docked L-arabinose are represented with cyan and purple sticks, respectively.