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. 1999 Apr;67(4):1623–1632. doi: 10.1128/iai.67.4.1623-1632.1999

FIG. 7.

FIG. 7

B. subtilis LTA and the reference LPS stimulated IL-1β secretion from human monocytes, and the stimulation was inhibited by S. sanguis LTA. (A) PBMC (2 × 105 cells/well) were stimulated with various doses of S. abortusequi LPS and LTAs from B. subtilis, S. sanguis, and S. mutans for 24 h. (B to D) PBMC (2 × 105 cells/well) were stimulated with 10 ng of S. abortusequi LPS per ml in the absence or presence of the indicated concentrations of S. sanguis LTA (B), LA-14-PP (C), or anti-CD14 MAb MY4 or isotype-matched mouse IgG2b (D) for 24 h. (E) PBMC (2 × 105 cells/well) were stimulated with 1 μg of B. subtilis LTA per ml in the absence or presence of the indicated concentration of S. sanguis LTA, LA-14-PP, MY4, or isotype-matched mouse IgG2b for 24 h. The amount of IL-1β in the supernatants was analyzed by an ELISA. Error bars indicate SD.