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Schematic diagram of the experimental principle and an image of the lung-on-a-chip. Fluorescence images and plots of fluorescence intensity profiles for characterization of small molecule diffusion in a microchip. Green, RH-123 aqueous solution. Red, RH-B aqueous solution. Scale bar = 1 mm. Effects of different concentrations of gefitinib, afatinib, and osimertinib on HCI-H1650 cells grown in the wells of a plate in 2D and in the 3D lung-on-a-chip. n = 3. Source: (Tan et al., 2022).
