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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Immunohorizons. 2022 Jul 25;6(7):528–542. doi: 10.4049/immunohorizons.2200050

FIGURE 1. Frequencies of Ag-experienced CD4 and CD8 T cells during the generation of SPexp mice.

FIGURE 1.

(A) Experimental design. Sequential infection of SPfree C57BL/6 mice with PR8, MCMV-Smith, vaccinia virus (VACV), attenuated L. monocytogenes (L.m.-att), and LCMV-Arm in 5-d intervals was used to generate SPexp mice. The frequency of Ag-experienced CD4 and CD8 T cells (CD3+CD4+CD11ahi and CD3+CD8loCD11ahi, respectively) in the PBLs was monitored every 5 d for 25 d. (B) Body weight changes were monitored during SPexp generation. Longitudinal analysis and representative gating (day 25 after PR8) of Ag-experienced (C) CD4 and (D) CD8 T cells. Prior gating on CD3+ single-cell lymphocytes. Numbers of Ag-experienced (E) CD4 and (F) CD8 T cells on days 0 and 25 after PR8 infection. Data are representative of at least three independent experiments with 5–10 mice per group. Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.