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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Immunohorizons. 2022 Jul 25;6(7):528–542. doi: 10.4049/immunohorizons.2200050

FIGURE 3. Immunologically experienced mice have exacerbated responses to sepsis.

FIGURE 3.

(A) Experimental design. Moderate septic event (<20% mortality in SPfree mice) was induced via CLP 5 d after last infection in SPexp and age-matched SPfree B6 mice. Weight loss and mortality were monitored over time, and serum cytokines were measured 12 h after CLP. Weight of mice (B) before and (C) after CLP. (D) Mortality of mice after CLP. (E) Summary data of analyte concentrations (pg/ml) in mice 12 h after CLP. (F) Individual serum IFN-γ, IL-1β, and TNF-α concentrations between mice 12 h after CLP. (G) Individual serum IFN-γ concentrations between SPfree and SPexp mice that survived or succumbed to CLP surgery. Data are representative of at least three independent experiments with 10 mice per group. (H) Experimental design. Five days after last infection, CLP was induced in SPfree and SPexp Swiss Webster mice. (I) Survival curves after CLP. Data are representative of at least three independent experiments with 10–15 mice per group. Error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001.