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. 2022 Nov 11;29:95. doi: 10.1186/s12929-022-00877-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Triple immunofluorescence staining of thrombin, IL-8/CXCL8 (MIP-2), and DCLK1. A Representative examples of triple immunofluorescence staining for thrombin, IL-8/CXCL8, and DCLK1 in bronchial biopsies from severe asthma patients compared to normal subjects (n = 4–5, original magnification = ×20), and B OVA-challenged compared to PBS-treated mice (n = 5–7, original magnification = ×20). The merged image demonstrates colocalization of thrombin, IL-8/CXCL8, MIP-2, and DCLK1. The Image J software was used to measure the mean fluorescence intensity (MFI) of thrombin, IL-8/CXCL8 (MIP-2), and DCLK1 from C severe asthma patients compared to normal subjects (n = 4–5, mean ± SEM, *p < 0.05 vs normal) and D OVA-challenged compared to PBS-treated mice (n = 5–7, mean ± SEM, *p < 0.05 vs PBS)