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. 2022 Nov 8;10:e14202. doi: 10.7717/peerj.14202

Figure 4. gCLCA2 is a multiple N-linked glycosylated protein and cleaved early in the medial golgi.

Figure 4

Left panel: illustration of protein cleavage and deglycosylation: scissor sketch, cleavage; Y, antibody binding site; PC, precursor protein; CC, C-terminal cleavage product; asterisk, deglycosylated, uncleaved precursor protein; hash, deglycosylated C-terminal cleavage product. Right panel: immunoblot analysis of lysates from HEK293 cells transfected with gCLCA2 or the EYFP-mock plasmids (C), using an anti-EYFP antibody. Lysates were treated with endoglycosidases endo H (H), PNGase F (F) or left untreated (U). To control for equal total protein loading, the samples were immunoblotted with anti-beta-actin antibodies (bottom panel). Representative image of three independent experiments.