Figure 3.

Gq inhibitor YM-254890 abrogates MCh-induced, but not PD 102807–induced, p-AMPK in human ASM cells expressing M3 mAChR. Human hTERT ASM cell lines were preincubated with YM-254890 (10 μM) for 15 min and then stimulated with (A) PD 102807 (100 μM; 20 min) or (B and C) MCh (100 μM; 5 min), and cell lysates were used for immunoblotting. Representative immunoblots for (A and B) p-AMPK and (C) p-ERK are shown; loading was corrected for β-actin. Data are means ± SEM from three to five experiments. *P < 0.05, **P < 0.01, and ****P < 0.0001 versus vehicle stimulation; ^##P < 0.01, and ^###P < 0.001 versus vehicle-pretreated, MCh-stimulated condition; one-way ANOVA followed by Bonferroni multiple comparison test. Veh = vehicle.