Table 1.
Cytokine Assessment in BALF and Lung Tissue Lysate
| Cytokine | VCD/HDM | VCD/Saline | Vehicle/HDM | Vehicle/Saline | |
|---|---|---|---|---|---|
| IgE (ng/mL + SEM) | 597.1 ± 110.3 *(versus VCD/Sal) | 36.3 ± 22.6 | 920 ± 236.20 ***(versus Veh/Sal) | 5.2 ± 3.7 | Serum |
| IL-4 (pg/mL + SEM) | 65.1 ± 8.4 **(versus VCD/Sal) | 32.8 ± 3.14 | 54.27 ± 6.3 *(versus Veh/Sal) | 29.2 ± 3.7 | BALF
|
| IL-5 (pg/mL + SEM) | 194.1 ± 28.7 ****(versus VCD/Sal) | 16.0 ± 3.9 | 134.1 ± 26.0 ***(versus Veh/Sal) | 8.6 ± 3.0 | |
| IL-13 (pg/mL + SEM) | 117.3 ± 16.2 | 83.0 ± 8.9 | 93.0 ± 14.5 | 74.9 ± 12.0 | |
| IL-4 (pg/mL + SEM) | 23.62 ± 5.96 *(versus VCD/Sal) | 4.45 ± 0.41 | 17.55 ± 7.73 | 3.35 ± 0.42 | Tissue
|
| IL-5 (pg/mL + SEM) | 214.0 ± 54.78 | 116.4 ± 6.04 | 194.6 ± 74.36 | 120.1 ± 59.2 | |
| IL-13 (pg/mL + SEM) | 155.0 ± 27.36 | 156.3 ± 19.11 | 153.0 ± 22.27 | 173.8 ± 19.16 | |
| IL-17A (pg/mL + SEM) | 2962 ± 1479 | 146.5 ± 19.4 | 1963 ± 1076 | 125.7 ± 17.44 | |
| IL-17F (pg/mL + SEM) | 1321 ± 94.99 *(versus Veh/Sal) | 1360 ± 195.7 *(versus Veh/Sal) | 1176 ± 275.5 | 457.5 ± 54.73 | |
| IL-22 (pg/mL + SEM) | 162.9 ± 55.56 | 57.62 ± 8.66 | 106.0 ± 47.28 | 61.28 ± 15.67 | |
| IL-6 (pg/mL + SEM) | 69.12 ± 4.86 *(versus Veh/HDM) | 46.7 ± 2.59 | 53.2 ± 3.14 | 52.25 ± 5.09 | |
| KC (pg/mL + SEM) | 136.9 ± 11.14 ***(versus Veh/HDM) | 57.92 ± 3.14 | 86.61 ± 5.67 | 74.7 ± 9.29 |
Cytokines were assessed in BAL fluid and lung lysates (tissue) by single or multiplex immunoassay kits; IgE was detected in serum by ELISA. Values shown are mean +/−SEM, n = minimum of 5 per group. Statistical analysis performed using one-way ANOVA for multiple comparisons.
*
P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.