TABLE 1.

Bacteria and recombinant plasmids used in this study

Bacterium or plasmid	Relevant genotype or description	Source or reference
K. pneumoniae IApc35	Strain lacking a functional mrkD gene; type 3 fimbriate and nonadhesive	22
E. coli HB101(pDC17)	Strain transformed with a plasmid carrying pap gene cluster lacking papG; P piliated and nonadhesive	8
Recombinant plasmids
pFK52	Plasmid carrying functional mrkD1P gene	8
pTS52	BamHI/HindIII DNA fragment carrying the mrkD1P gene cloned into pBluescript KS	This study
pTS68, pTS71, pTS72, pTS76	Derivatives of pFK52 carrying site-directed mutations in mrkD1Pa	This study
pTS73, pTS74, pTS78, pTS79	Derivatives of pFK52 carrying hydroxylamine-induced mutations in mrkD1Pa	This study

^aSites of the mutations are indicated in Fig. 1.