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Oligonucleotides used to construct site-specific mutations
| Nucleotide sequencea | Site(s) (change in amino acid sequence) | Plasmid carrying mutation |
|---|---|---|
| ATTGGTTTAGCATTCTCAGCAGCAGGGGCGATCAGTATG | 101, 104–105 (R, R-K)b | pTS68 |
| CTCCATGCGCGCTTCGCAGCAGCAGCGCCGACTACCAGT | 299–301 (Y-Q-Y) | pTS71 |
| GATATAGGCACGATTGCAGCAGCTGCATTAAAAGGGGTGGGG | 194–195, 197 (K-R, D) | pTS72 |
| ATTTATGTTACATGTGCAGCAGCAACTACTCTGAAATCA | 68–70 (D-R-N) | pTS76 |
Sites of mutations are underlined.
Amino acids changed to alanine.
