TABLE 3.
Transformant | MR/K HA | MR/K HA titera | Presence of MrkD on fimbriaeb |
---|---|---|---|
E. coli(pDC17) | |||
pFK52 | +c | 5.8 × 107 | ND |
pTS68 | − | −d | ND |
pTS71 | − | − | ND |
pTS72 | − | − | ND |
pTS73 | − | − | ND |
pTS74 | − | − | ND |
pTS76 | + | 9.4 × 108 | ND |
pTS78 | − | − | ND |
pTS79 | − | − | ND |
pTS80 | − | − | ND |
K. pneumoniae IApc35 | |||
pFK52 | + | 2.1 × 107 | + |
pTS68 | − | ND | − |
pTS71 | − | ND | − |
pTS72 | − | ND | − |
pTS73 | − | ND | + |
pTS74 | − | ND | + |
pTS76 | + | 6.3 × 108 | + |
pTS78 | − | ND | + |
pTS79 | − | ND | + |
pTS80 | − | ND | + |
Titer expressed as the lowest concentration (in CFU per milliliter) of bacteria causing visible HA.
Presence of adhesin as determined by Western blot analysis of purified fimbrial preparations (see Materials and Methods). ND, not determined.
+, present; −, absent.
Bacterial concentrations of >1.5 × 1010 CFU ml−1 did not cause HA.