TABLE 3.
Fimbriation and MR/K HA activity of transformants
| Transformant | MR/K HA | MR/K HA titera | Presence of MrkD on fimbriaeb |
|---|---|---|---|
| E. coli(pDC17) | |||
| pFK52 | +c | 5.8 × 107 | ND |
| pTS68 | − | −d | ND |
| pTS71 | − | − | ND |
| pTS72 | − | − | ND |
| pTS73 | − | − | ND |
| pTS74 | − | − | ND |
| pTS76 | + | 9.4 × 108 | ND |
| pTS78 | − | − | ND |
| pTS79 | − | − | ND |
| pTS80 | − | − | ND |
| K. pneumoniae IApc35 | |||
| pFK52 | + | 2.1 × 107 | + |
| pTS68 | − | ND | − |
| pTS71 | − | ND | − |
| pTS72 | − | ND | − |
| pTS73 | − | ND | + |
| pTS74 | − | ND | + |
| pTS76 | + | 6.3 × 108 | + |
| pTS78 | − | ND | + |
| pTS79 | − | ND | + |
| pTS80 | − | ND | + |
a
Titer expressed as the lowest concentration (in CFU per milliliter) of bacteria causing visible HA.
b
Presence of adhesin as determined by Western blot analysis of purified fimbrial preparations (see Materials and Methods). ND, not determined.
c
+, present; −, absent.
d
Bacterial concentrations of >1.5 × 1010 CFU ml−1 did not cause HA.