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Binding of 125I-labelled hLf to S. pneumoniae in the presence of various concentrations of unlabelled hLf. Equilibrium binding of hLf to pneumococcal strain type 3 (NCTC 7978) with 23.6 ng of 125I-labelled hLf and increasing concentrations of unlabelled hLf is shown. The data were also plotted as described by Scatchard (19) (inset). Values shown are the means of triplicate determinations.
