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Analysis of hLf binding and protease sensitivity of the purified hLf-binding receptor from S. pneumoniae. The purified pneumococcal hLf-binding receptor was treated with proteases and neuraminidase. (A) Silver-stained samples; (B) immunoblot analysis with hLf. Lanes: 1, untreated purified hLf protein; 2, 30-min incubation at 37°C without enzyme; 3, trypsin-treated sample; 4, 15-min incubation at 37°C without enzyme; 5, pronase E-treated sample; 6, neuraminidase-treated sample.
