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. 2022 Jul 8;119(28):e2122840119. doi: 10.1073/pnas.2122840119

Fig. 2.

Fig. 2.

Low GGT1 is associated with high expression of GCLC and GPX4 in ChRCC. (A) GPX4 mRNA level in ChRCC compared with normal kidney from TCGA. (B) GPX4 mRNA level in ccRCC compared with normal kidney from TCGA. (C and E) qRT-PCR analyses of GCLC (C) and GPX4 (E) expression in UOK276 and HEK293T cells with the indicated expression of EGFP or GGT1 or with siRNA for GGT1 (n = 3 biological replicates per condition). (D and F) GCLC and GPX4 protein expression in UOK276 cells with EGFP or GGT1 expression in vitro (D) and in vivo (F). (G) Quantitation of GCLC and GPX4 protein expression from F using Image J. Error bars represent mean ± SD. Statistical analyses were performed using two-tailed Student’s t test or one-way ANOVA if more than two groups. **P < 0.01; ****P < 0.0001.