Fig. 6. Characterization of post–second dose inflammation, erythroid cell, and plasmablast responses.

(A) The bar graph at the top represents the number of responsive modules at any given time point after the first and second doses. The fingerprint grid plots below map the modular response observed on day 1 after the second dose (left) and day 4 after the second dose (right). (B) The line graphs show the average percentage responses of A35, A37, and A27 modules across multiple time points (left, middle, and right, respectively). Each line represents the profile of the modules constituting a given aggregate. For all line graphs, centerlines, box limits, and whiskers represent the mean, interquartile range, and 1.5× interquartile range, respectively. The significance of changes measured after the second dose was determined at each time point and is shown on the graphs (paired t test comparing module response at each time point relative to the prevaccination baseline; *P < 0.01, **P < 0.001, and ***P < 0.0001). (C) The heatmaps represent associations between levels of module response measured at the prevaccination baseline (S0) and for each of the time points after the second dose (S1 to S9 and S14) and SARS-CoV-2 S1–specific antibody levels measured at the prevaccination baseline (S0) and at 14 days after the second dose (S14). Specifically, the heatmap at the top (blue-red color gradient) represents the correlation coefficients across multiple days and for each day across multiple subjects, with rows corresponding to the five A27 plasmablast modules. The heatmap below (green color gradient) represents the significance of the correlations shown on the heatmap at the top, with the same order of rows and columns.