Fig. 7.

Effects of NPs on the induction of apoptosis. A Fluorescence microscopy images B FACS of Annexin V/PI double staining in MCF-7 cells treated for 48 h with PCL NPs (negative control), MUC1i-PEG-PCL NPs at 43.3 nM, DOX-PEG-PCL NPs at 25.7 nM, and DM-PEG-PCL NPs at 5.8 nM. (BD Biosciences, San Jose, California).C Immunoblotting D quantify immunoblots for 48 h, MCF-7 cells were given PEG-PCL NPs (negative control), MUC1i-PEG-PCL NPs at 50 nM, DOX-PEG-PCL NPs at 31.4 nM, and DM-PEG-PCL NPs at 11.5 nM. Immunoblotting with the identified antibodies was used to examine whole cell lysates and quantification through Image J software