Figure 3. Effects of fungal β-GPs on CEACAM1 mRNA expression in RT7 cells.

(A) Cells were incubated with C. albicans β-glucan-containing particles (CA β-GPs) (200 μg/mL) or heat-killed (108/mL) C. albicans (CA) for 12 hours. Gene mRNA levels are presented as relative to that of β-actin. Then, RT-PCR assays were performed for CEACAM1 and β-actin. The experiments were performed at least three times, with representative results shown.

(B) Cells were incubated with CA β-GPs (200 μg/mL) or S. cerevisiae β-GPs (SC β-GPs) (200 μg/mL) for 12 hours. Gene mRNA levels are presented as relative to that of β-actin. Values are shown as fold increase as compared with non-treated cells and presented as the mean ± SD of three independent experiments. *Significantly different from non-treated control (Bonferroni/Dunn's multiple comparison test: p<0.05).