Figure 8. Effects of knockdown of CEACAM1 on β-GPs-induced reactive oxygen species in RT7 cells.

Cells were transfected with CEACAM1 siRNA for 48 hours, then labelled with DCF-DA for 30 minutes and stimulated with C. albicans β-glucan-containing particles (CA β-GPs) (200 μg/mL) for 1 hour. Fluorescence intensity was then determined. Values are shown as fold increase as compared with the intensity of Si control cells and presented as the mean ± SD of five independent experiments. #Significantly different from Si control-transfected cells stimulated with C. albicans β-GPs alone (Bonferroni/ Dunn's multiple comparison test, p<0.05).