Figure 9. Effects of knockdown of CEACAM1 on β-GPs-induced translocation of Nrf2 into nucleus in RT7 cells.

Cells were transfected with siRNA for CEACAM1 siRNA for 48 hours, then stimulated with C. albicans β-glucan-containing particles (CA β-GPs) (200 μg/mL) for 4 hours. Whole cell and nuclear extractions were prepared, then subjected to western blot analysis with the Nrf2 antibody. LaminB and GAPDH were used as marker proteins for the nuclear and whole cell fractions, respectively. The experiments were performed at least three times, with representative results shown.