Video 4. Representative example of identifying SAG to pC1 connections via the SAG presynaptic site contact with the pC1 membrane.

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SAG and pC1 were labeled by membrane reporters. SAG and pC1 were semiautomatically segmented, and neuron masks were generated. Presynaptic sites labeled by BRP were automatically classified and assigned to SAG. Connections between SAG presynaptic sites and the pC1 neuron mask were automatically identified.