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. 2022 Nov 11;86:104341. doi: 10.1016/j.ebiom.2022.104341

Fig. 2.

Fig. 2

Characterization of S2 and VLP-S2.(a) SDS-PAGE characterization of S2 and VLP-S2. S2 was deglycosylated with PNGase F. The samples were heated with β-mercaptoethanol and LDS sample buffer. The unprocessed gel is shown in Fig. S2a. (b) Size exclusion chromatography traces for S2 (dashed line) and VLP-S2 (solid line). The vertical gray line represents the peak elution volume of the molecular weight standard thyroglobulin (660 kDa). The column void volume is 7.2 mL. (c) Characterization of the VLP-S2 by dynamic light scattering. (d) Negative-stain transmission electron micrograph of VLP-S2. Arrowheads (white) indicate the S2 protein on the VLP surface. Scale bars = 50 nm. (e) Cryo-EM of vitrified VLP-S2. Arrowheads (white) indicate the S2 protein on the VLP surface. Scale bars = 50 nm. (f) Characterization of the binding of anti-S2 antibody 0304-3H3 to S2 and VLP-S2 by ELISA. (mean ± SD, n = 6: two independent assays, each with three technical replicates).