Table 3.

Antibody profile of the anti-CD147 antibody, h4^#147D.

Name of the antibody	h4#147D
Method of antibody generation for the parental nonhuman antibody	Myeloma fusion with rat lymphocytes derived from immunized rat with recombinant human CD147 protein
IgG subtype after humanization	Human IgG4 S228P (with deficiency in inter-H-chain bonds can be corrected by a single mutation: changing Ser228 (EU numbering) to pro [32])
Affinity to human CD147 (KD, SPR)	8.6 nM
Affinity to monkey CD147 (KD, SPR)	5.8 nM
Summary of epitope mapping using X-ray crystallographic structure	Hinge to the membrane-proximal immunoglobulin domain of CD147 isoform 2 (Arg106, Lys108 to Val110, Lys127 to Val134, and Gln164 to Gly165 in the amino acid sequence of human CD147 referred for Uniprot ID : P35613-2)