FIG. 2.

CD4⁺ Th cell responses (A) and 4E6 T-cell hybridoma responses (B) to SDS-PAGE fractions on immunoblots of SEA. CD4⁺ Th cells were isolated from mesenteric lymph nodes of 8.5-week-infected BL/6 mice. Cell culture conditions in each case were as described in Materials and Methods. [³H]dThd incorporation was assessed by liquid scintillation spectroscopy. Data are expressed as means ± 1 SD. Background radioactivity from cultures in the absence of antigen is subtracted. Panels on the right reflect corresponding CD4⁺ Th cell or hybridoma stimulation in the presence of NC, NC coated with 50 μg of SEA (SEA-NC), or the indicated amounts of SEA. Experiments with CD4⁺ Th cells as well as with the T-cell hybridoma were repeated twice, with similar results. Frs., fractions.