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. 2022 Oct 28;13:1034648. doi: 10.3389/fimmu.2022.1034648

Figure 2.

Figure 2

Expression of GPR183 or CH25H in Rag-/- mice are not required for colitis. Colitis was induced by adoptive transfer of naive CD4+ T cells from wildtype C57BL/6 mice into GPR183 deficient (Gpr183-/- ) vs. littermate control (Gpr183+/- ) Rag1-/- recipients. (A) Weight change was monitored as % starting body weight on the indicated days. A representative weight graph from one of 3 independent experiments is shown. (B) H&E staining of representative colon sections (middle part of whole colon) at 40x (scale bar 100 μm) magnification. (C) Blinded histology score from the middle part of whole colon combined from 3 independent experiments. Representative images in B) were taken from mice represented by the orange colored data points. (D) Colitis was induced by adoptive transfer of naive CD4+ T cells from wildtype C57BL/6 mice into CH25H deficient (Ch25h-/- ) vs. control (Ch25h+/- ) Rag1-/- recipients. Disease progression was monitored by assessing weight loss. A representative weight graph from one of 3 independent experiments is shown. The weight of dead mice was carried forward, indicated with + signs in magenta for control mice and in black for Rag1-/-Ch25h-/- mice. (E) Colon explant cytokine quantification from distal colon at the end of T-cell transfer model. Cytokines were measured by CBA; two independent experiments were pooled. Error bars represent S.E.M. Two-way ANOVA (Tukey’s test) (A and D) and nonparametric T-test (Mann-Whitney test) (C) and One-way ANOVA (Tukey’s test) (E). ns, not significant.